In fact, several studies have observed increased cortisol in plasma and adrenal glands of mice following stress, and many studies have even used cortisol as the index for stress activation in both mice and rats. Although corticosterone is considered the main glucocorticoid involved in regulation of stress responses in rodents, researchers often choose to detect cortisol for stress indicators in consideration of convenience and kits availability. Cortisol and corticosterone are thus often used as biomarkers for stress and depressive disorders. It is known that stress enhances the activity of the hypothalamus-pituitary-adrenal (HPA) axis and results in increased secretion of corticosteroids from the adrenal cortex. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: Co-author Jing-He Tan is a PLOS ONE Editorial Board member, but this does not alter the authors' adherence to PLOS ONE Editorial policies and criteria. 2014CB1385CB944403) and the China National Natural Science Foundation (Nos. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedĭata Availability: All relevant data are within the paper.įunding: This study was supported by grants from the National Basic Research Program of China (Nos. Received: AugAccepted: DecemPublished: February 20, 2015Ĭopyright: © 2015 Gong et al.
PLoS ONE 10(2):Īcademic Editor: Andrew Wolfe, John Hopkins University School of Medicine, UNITED STATES (2015) Dynamics and Correlation of Serum Cortisol and Corticosterone under Different Physiological or Stressful Conditions in Mice. Taken together, results (i) confirmed the presence of cortisol in mouse serum and (ii) suggested that mouse serum cortisol and corticosterone are closely correlated in dynamics under different physiological or stressful conditions, but, whereas corticosterone was a more adaptation-related biomarker than cortisol during chronic stress, cortisol was a quicker responder than corticosterone during severe acute stress.Ĭitation: Gong S, Miao Y-L, Jiao G-Z, Sun M-J, Li H, Lin J, et al. Analysis with HPLC and HPLC-MS further confirmed the presence of cortisol in mouse serum. During forced swimming or heat stress, whereas cortisol increased to the highest level within 3 min., corticosterone did not reach maximum until 40 min. Thus, whereas corticosterone declined dramatically during repeated restraints, it remained at the high level during unpredictable stress. Both hormones increased to the highest level on day 1 of repeated-restraint or unpredictable stresses, but after that, whereas the concentration of cortisol did not change, that of corticosterone showed different dynamics. A strong (r = 0.6–0.85) correlation between serum cortisol and corticosterone was observed throughout the estrous cycle, all day long, and during acute or repeated restraints, chronic unpredictable stress and acute forced swimming or heat stress. In this study, effects of estrous cycle stage, circadian rhythm, and acute and chronic (repeated or unpredictable) stressors of various severities on dynamics and correlation of serum cortisol and corticosterone were examined in mice. Although plasma corticosterone is considered the main glucocorticoid involved in regulation of stress responses in rodents, the presence of plasma cortisol and whether its level can be used as an indicator for rodent activation of stress remain to be determined.
0 kommentar(er)
